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Following the staining, cells were washed three times with HBSS to remove excess probe, mounted onto a microscope slide, and immediately imaged using a Carl Zeiss LSM-900 confocal laser scanning microscope

Adv Drug Deliv Rev 97:4155

The metabolic gene signature might also explain the differential efficacy of gemcitabine over pemetrexed in SCC compared to AdC when paired with cisplatin 58 and the preferred choice of combined gemcitabine and a platinum-based drug for first-line treatment of SCC patients

To examine whether the compounds had specific effects, we retested them at varying concentrations and confirmed the zebrafish phenotypes

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